世联博研公司现货供应bpsbioscience品牌订货号为79757的MLL4 Complex Chemiluminescent Assay Kit

产品名称

MLL4 Complex Chemiluminescent Assay Kit - Methyltransferase(s)

产品货号

79757

产品价格

现货询价，电话：010-67529703

产品规格

96 reactions

产品品牌

bpsbioscience

产品概述

产品详情

Description:

The MLL4 Complex Chemiluminescent Assay Kit is designed to measure MLL4 activity for screening and profiling applications, using purified MLL4 and its complex components: WDR5, RbBP5, Ash2, and DPY30. The key to the MLL4 Complex Chemiluminescent Assay Kit is a highly specific antibody that recognizes methylated K4 residue of Histone H3. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme. Next, primary antibody is added. Finally, the plates are treated with an HRP-labeled secondary antibody followed by the addition of the HRP substrate to produce chemiluminescence that can be measured using a chemiluminescence reader.

Materials Required But Not Provided:

TBST buffer (1 x TBS, pH 8.0, containing 0.05% Tween-20)
Luminometer or fluorescent microplate reader capable of reading chemiluminescence
Adjustable micropipettor and sterile tips
Rotating or rocker platform
0.5 M DTT (dithiothreitol)

Synonym(s):

KMT2B, HRX2, MLL1B, MLL2, MLL4, TRX2, WBP-7, WBP7, CXXC10, lysine methyltransferase 2B, DYT28

Supplied As:

The MLL4 Complex Chemiluminescent Assay Kit comes in a convenient format, with 8-well strips pre-coated with histone H3 peptide substrate, an antibody against methylated lysine on Histone H3, a secondary HRP-labeled antibody, S-adenosylmethionine, methylt

Format:

Catalog #	Component	Amount	Storage
100324	MLL4/WDR5/Ash2L/RbBP5/DPY30	3 µg	-80°C	*Avoid freeze/ thaw cycles!*
52120	20 µM S-adenosylmethionine	250 µl	-80°C
52140Z	Primary antibody 26	12.5 µl	-80°C
52160	4x HMT assay buffer 1*	3 ml	-20°C
52131H	Secondary HRP-labeled antibody 2	10 µl	-80°C
52100	Blocking buffer	50 ml	+4°C
79670	ELISA ECL Substrate (2 components)	6 ml each	+4°C
	96-well strip well plate pre-coated with histone substrate	1 plate	+4°C

*Add 125 µl of 0.5 M DTT before use.

Instructions for use:

See assay kit protocol for detailed instructions.

Storage / Stability:

Up to one year from date of receipt when stored as directed.

Application(s):

Great for studying enzyme kinetics and HTS applications.

Reference(s):

Dillon, S.C., et al. Genome Biology 2005; 6:227.

Warning(s):

Avoid freeze/thaw cycles

Scientific Category:

Methyltransferase

Product Type:

Assay Kit

Data shown is lot-specific. Contact us for specific information on other lots.

Qty:

Quality Assurance

Product Datasheets

79757.pdf

产品资料