The METTL3/METTL14 Complex Chemiluminescent Assay Kit is designed to measure the METTL3/METTL14 complex activity for screening and profiling applications. The METTL3/METTL14 Complex Chemiluminescent Assay Kit comes in a convenient format, with 96-well plate precoated with a specific substrate, the antibody against methylated adenosine, a secondary HRP-labeled antibody, S-adenosylmethionine, methyltransferase assay buffer, and purified METTL3/METTL14 complex enzyme for 96 enzyme reactions. The key to the METTL3/METTL14 Complex Chemiluminescent Activity Assay Kit is a highly specific antibody that recognizes N6methylated adenosine. With this kit, only three simple steps are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.
| 产品名称 | METTL3/METTL14 Complex Chemiluminescent Assay Kit - Methyltransferase(s) |
| 产品货号 | 79614 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | 96 Reactions |
| 产品品牌 | bpsbioscience |
| 产品概述 | |
| 产品详情 |
Description: Materials Required But Not Provided: TBST buffer (1 x Tris-buffered saline (TBS), pH 8.0, containing 0.05% Tween-20),
Luminometer or fluorescent microplate reader capable of reading chemiluminescence,
Adjustable micropipettor and sterile tips,
Rotating or rocker platform.
Synonym(s): Methyltransferase-like 3, METTL3, RNA, METTL14
Supplied As: The METTL3/METTL14 Complex Chemiluminescent Assay Kit comes in a convenient format, with 96-well plate precoated with a specific substrate, the antibody against methylated adenosine, a secondary HRP-labeled antibody, S-adenosylmethionine, methylt
Contraindications: DMSO >1%, strong acids or bases, ionic detergents, high salt.
Format: COMPONENTS:
Instructions for use: See assay kit data sheet for detailed protocol.
Storage / Stability: One year from date of receipt when stored as directed.
Application(s): Great for studying enzyme kinetics and HTS applications.
Reference(s): Liu, J., et al. Nat Chem Biol. 2014; 10(2): 93–95. Warning(s): Avoid freeze/thaw cycles. Scientific Category: Methyltransferase
Product Type: Assay Kit
Data shown is lot-specific. Contact us for specific information on other lots.
|
| 产品资料 |
