| 产品名称 | G9a (EHMT2), GST-tag (Sf9-derived) |
| 产品货号 | 51001 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | 20 µg |
| 产品品牌 | bpsbioscience |
| 产品概述 | |
| 产品详情 |
Species: Human
Host Species: Sf9 insect cells
MW: 74.6 kDa
Genbank #: NM_006709
Tag(s): N-terminal GST-tag
a.a: 785–1210
Background: EHMT2 (G9a) is a lysine-preferring histone methyltransferase. EHMT2 can methylate itself at K165 and this automethylation is necessary and sufficient to mediate in vivo interaction of EHMT2 with heterochromatin protein-1 (HP1). EHMT2 is responsible for the majority of dimethylation of histone H3 at lysine 9 (H3K9me2) and is required for the efficient repression of developmentally regulated genes during embryonic stem cell differentiation. EHMT2 is overexpressed in various cancers and can can specifically methylate p53 at Lys(373). EHMT2 polymorphisms have been associated with colorectal cancer.
Description: Human G9a, also known as EHMT2 (euchromatic histone-lysine N-methyltransferase 2) and KMT1C (GenBank Accession No. NM_006709), a.a. 785-1210, with N-terminal GST-tag, MW = 74.6 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
UniProt Q96KQ7
Synonym(s): G9a, EHMT2, KMT1C, euchromatic histone-lysine N-methyltransferase 2
Specific Activity: ≥70 pmol/min/µg
Assay Conditions: 50 μl
reaction mix (50 mM TRIS pH 8.8, 5mM MgCl2,
4mM DTT, 0.05% Tween-20, 40 μM S-
adenosylmethionine, and G9a (0.05 – 0.5 ng)
add to the wells coated with the substrate on a
GBioscience Neutravidin black plate. Incubate
for 1 hr. Add antibody against methylated K9
residue of histone H3, incubate 1 hr. Then, add
secondary HRP-labeled antibody and incubate
30 min. Finally, add HRP chemiluminescent
substrates and read luminescence.
Formulation: 45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 Mm KCl, 3 mM DTT and 10% glycerol
Format: Aqueous buffer solution
Storage / Stability: >6 months at -80°C. Application(s): Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s): 1. Yuan,X. et al., Mol. Cell 27, 4, 585-595, 2007. Application Reference(s):
Warning(s): Avoid freeze/thaw cycles
Scientific Category: Methyltransferase
Product Type: Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
|
| 产品资料 |
