| 产品名称 | PRMT8, His-FLAG-tags |
| 产品货号 | 51052 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | 20 µg |
| 产品品牌 | bpsbioscience |
| 产品概述 | |
| 产品详情 |
Species: Human
Host Species: Sf9 insect cells
MW: 40.8 kDa
Genbank #: NM_019854
Tag(s): N-terminal His-FLAG-tags
a.a: 61–394(end)
Background: Membrane-associated arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and asymmetrical dimethylarginine (aDMA). Able to mono- and dimethylate EWS protein; however its precise role toward EWS remains unclear as it still interacts with fully methylated EWS.
Description: Human PRMT8 (protein arginine methyltransferase 8), GenBank Accession No. NM_019854, a.a. 61-394(end) with N-terminal His-FLAG-tag, MW=40.8 kDa, expressed in Sf9 cells using a Baculovirus expression system.
UniProt Q9NR22
Synonym(s): protein arginine N-methyltransferase 8, PRMT8, Histone-arginine N-methyltransferase 8, HRMT1L3, HRMT1L4
Specific Activity: 2.22 pmol/min/µg
Assay Conditions: 50 µl reaction mix (20 mM phosphate buffer pH 7.4, 20 µM S-adenosyl methionine, and 1-5 ng methyltransferase PRMT8) added to the wells coated with the substrate. Incubate at RT for 1 hr. Then, add secondary HRP-labeled antibody and incubate 30 min. Finally,add HRP chemiluminescent substrates and read luminesence.
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 80 µg/ml FLAG peptide, 3 mM DTT, 20% glycerol.
Format: Aqueous buffer solution
Storage / Stability: >6 months at -80°C. Application(s): Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s): 1. Bedford MT., et al. J Biol Chem. 2007 Dec 14;282(50):36444-53.
2. Taneda T., et al Brain Res. 2007 Jun 25;1155:1-9. Warning(s): Avoid freeze/thaw cycles
Scientific Category: Methyltransferase
Product Type: Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
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