产品名称 Histone H3 Dimethyl-K27 ELISA Detection Kit
产品货号 53030
产品价格 现货询价,电话:010-67529703
产品规格 96 reactions
产品品牌 bpsbioscience
产品概述
产品详情
Background:
Abnormal methylation of H3K27 is associated with a number of cancers, including breast, colorectal, and Non-Hodgkins lymphoma. The wild-type enzymatic activity of the EZH2 complex modifies Lysine 27 with two methyl groups while mutated forms of the EZH2 complex have altered activity.
Description:
The Histone H3 Dimethyl-K27 Detection Kit is designed to detect dimethylation of lysine 27 of Histone H3 (H3K27me2) in cell lysates. H3K27 is the substrate of the EZH2 methyltransferase complex. The kit comes in a convenient format, with a 96-well plate for coating with cell lysate, a detection antibody against the dimethylated K27 residue of Histone H3, and HRP labeled substrates.
Format:

COMPONENTS:

Instructions for use:
See product datasheet for detailed protocol.
Storage / Stability:
One year from date of receipt when stored as directed.
Application(s):
Detection of H3K27(Me2) in cell lysates
Notes:

MATERIALS OR INSTRUMENTS REQUIRED BUT NOT SUPPLIED:
Luminometer or fluorescent microplate reader capable of reading chemiluminescence
Rotating or rocker platform
RIPA lysis buffer (Pierce, #89900) with protease inhibitor (PI) cocktail (1x) and 1 mM Phenylmethanesulfonyl fluoride (PMSF)
BCA Protein Assay Kit (Pierce, #23227)

Scientific Category:
Methyltransferase
Product Type:
Assay Kit
Data shown is lot-specific. Contact us for specific information on other lots.

Quality Assurance

Product Datasheets

53030.pdf
产品资料