Human Histone 3 (HIST1H3E), GenBank Accession No. NM_003532, a.a. 2-136(end) with K27M mutation and N-terminal His-tag, MW = 16.2 kDa, expressed in an E. coli expression system.

P68431

Histone 3.1

8 mM PBS pH 7.4, 110 mM NaCl, 2.2 mM KCl and 20% glycerol

Aqueous buffer solution

At least 6 months at -80°C

Useful as a substrate for methyltransferases or acetyltransferases and for screening inhibitors and selectivity profiling for drug discovery.

1. Loyola, A., et al., Mol. Cell. 2006; 24(2): 309-316.
2. Khuong-Quang, D-A., et al., Acta. Neuropathol. 2012; 10.1007/s00401- 012-0998-0.

Application Reference(s):
Differential mode of antimicrobial actions of arginine-rich and lysine-rich histones against Gram-positive Staphylococcus aureus (2013)

Avoid freeze/thaw cycles. Storing diluted protein is not recommended, if necessary, use carrier protein (BSA 0.1 – 0.5%).

Methyltransferase

Recombinant Protein