The PRMT7 Homogeneous Assay Kit (Automethylation) is designed to measure PRMT7 automethylation for screening and profiling applications. Automethylation of PRMT7 plays a key role in inducing the epithelial–mesenchymal transition program and in promoting the migratory and invasive behavior of breast cancer cells. The key to the PRMT7 Homogeneous Assay Kit (Automethylation) is a highly specific antibody that recognizes methylated PRMT7. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing PRMT7 enzyme is incubated with SAM. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.

Note: To study methylation of histone proteins by PRMT7, we recommend using our PRMT7 Homogeneous Assay Kit (H2b), #79720.

AlphaLISA® anti-rIgG acceptor beads, 5 mg/ml (PerkinElmer #AL104C)

AlphaScreen® Flag donor beads, 5 mg/ml (PerkinElmer #AS103D)

Optiplate-384 (PerkinElmer #6007290)

Dithiothreitol (DTT), 0.5 M

Protein Arginine Methyltransferase 7, [Myelin Basic Protein]-Arginine N-Methyltransferase PRMT, Histone-Arginine N-Methyltransferase PRMT7, Protein Arginine N-Methyltransferase 7, EC 2.1.1.321, KIAA1933, EC 2.1.1, SBIDDS

The PRMT7 Homogeneous Assay Kit (Automethylation) comes in a convenient AlphaLISA® format, with S-adenosylmethionine (SAM), primary antibody, methylation assay buffer, and purified Flag-tagged PRMT7 for 384 enzyme reactions.

Green and blue dyes that absorb light in the AlphaScreen® signal emission range (520-620 nm), such as Trypan Blue. Avoid the use of the potent singlet oxygen quenchers such as sodium azide (NaN3) or metal ions (Fe2+, Fe3+, Cu2+, Zn2+ and Ni2+). The presence of >1% RPMI 1640 culture medium leads to a signal reduction due to the presence of excess biotin and iron in this medium. MEM, which lacks these components, does not affect AlphaScreen® assays.

See assay protocol for detailed instructions.

At least one year from date of receipt when stored as directed.

Great for studying enzyme kinetics and HTS applications.

1. You Feng, et al. 2014. J Biol Chem 289:32604.
2. Pengyu Geng, et al. 2017. FASEB J. 31:2287.

Avoid multiple freeze/thaw cycles.

Methyltransferase

Assay Kit