ChIP results obtained with the antibody directed against EZH2
ChIP assays were performed using HeLa cells, the antibody against EZH2 (Cat. # 25223) and optimized PCR primer sets for qPCR. ChIP was performed using sheared chromatin from 1 million cells. A titration of the antibody consisting of 0.5, 1, 2.5 and 5 µg per ChIP experiment was analyzed. IgG (2 µg/IP) was used as negative IP control. Quantitative PCR was performed with primers for MYT1, used as a positive control target, and for the promoter of the active GAPDH gene, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

Western blot analysis using the antibody directed against EZH2
Nuclear extracts of HeLa cells (40 µg) were analyzed by Western blot using the antibody against EZH2 (Cat. # 25223) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest (expected size 85 kDa) is indicated on the right; the marker (in kDa) is shown on the left. 

Store at –80°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze/thaw cycles. Aliquots may be stored at –20°C for at least one month.