The EZH2 (Y641F) TR-FRET Assay Kit is designed to measure activity of the mutant EZH2 complex (EZH2 (Y641F)/EED/SUZ12/RbAp48/ AEBP) in a homogeneous 384 reaction format. This FRET-based assay requires no timeconsuming washing steps, making it especially suitable for high throughput screening applications. The EZH2 (Y641F) TR-FRET Assay Kit comes in a convenient format, with histone H3 peptide substrate, a Tb-labeled antibody against methylated K27 residue of Histone H3, S-adenosylmethionine, methyltransferase assay buffer, TR-FRET detection buffer, dye-labeled acceptor, and purified EZH2 (Y641F) complex for 384 enzyme reactions. The key to the EZH2 (Y641F) Assay Kit is a highly specific antibody that recognizes methylated Histone H3K27. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for 240 minutes. Next, antibody is added. Finally, dye-labeled acceptor is added followed by fluorescence reading.

Q15910

EZH2 (Y641F), EED, SUZ12, RbAp48, AEBP, HTRF

The EZH2(Y641F) TR-FRET Assay Kit comes in a convenient format for 384 enzyme reactions.

See assay kit data sheet for detailed protocol

Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Great for screening small molecular inhibitors for drug discovery and HTS applications.

1. Dillon, S.C., et al. Genome Biology 2005; 6:227.

2. Morin, R.D., et al. Nat Genet. 2010, 42(2):181.

Avoid freeze/thaw cycles.

Methyltransferase

Assay Kit