Determination of the antibody titer
To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against human DNMT3A (Cat. # 25218), crude serum and flow-through in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:2,000.

Western blot and Immunoprecipitation using the antibody directed against DNMT3A
Human embryonic kidney cells (HEK293T) were transiently transfected with an expression vector for GAL4-tagged DNMT3A. Whole cell extracts were immunoprecipitated with 2 µg of antibody against DNMT3A (Cat. # 25218). The presence of GAL4-DNMT3A in the non-treated cell extracts and in the immunoprecipitates was demonstrated by Western blot analysis with anti-GAL4 antibody (lane 1 and 2 respectively). Alternatively, GAL4-DNMT3A was immunoprecipitated and Western blot analysis was performed with the DNMT3A antibody (diluted 1:2,500) (lane 3).

Store at –80°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze/thaw cycles. Aliquots may be stored at –20°C for at least one month.