| 产品名称 | ERK2, GST-tag |
| 产品货号 | 40299 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | 10 µg |
| 产品品牌 | bpsbioscience |
| 产品概述 | |
| 产品详情 |
Species: Human
Host Species: E. coli
MW: 68 kDa
Genbank #: NM_002745
Tag(s): N-terminal GST-tag
a.a: full length
Background: ERK2 is a protein serine/threonine kinase that is a member of the extracellular signal-regulated kinases (ERKs) which are activated in response to numerous growth factors and cytokines (1). Activation of ERK2 requires both tyrosine and threonine phosphorylation that is mediated by MEK. ERK2 is ubiquitously distributed in tissues with the highest expression in heart, brain and spinal cord. Activated ERK2 translocates into the nucleus where it phosphorylates various transcription factors (e.g., Elk-1, c-Myc, c-Jun, c-Fos, and C/EBP beta).
Description: Human ERK2, also known as MAPK1 or MAPKp42, GenBank Accession No. NM_002745, full length, with N-terminal GST-tag, MW ~68 kDa, expressed in E. coli and activated by MEK1 in vitro.
Synonym(s): MAPK1, P42MAPK, MAPK2, PRKM2, PRKM1, p41mapk, ERT1, p40, p41, ERK
Purity: ≥90%
Specific Activity: 682 pmol/min/µg
Assay Conditions: 5 mM MOPS, 2.5 mM β-glycerophosphate, 5 mM MgCl2, 1 mM EGTA, 0.4 mM EDTA, 0.05 mM DTT, 2 mM ATP. Incubate ERK2 with 0.25 mg/ml Myelin basic Protein (substrate) and 50 ?M [33P]-ATP at 30°C for 15 minutes, then spot reaction on phosphocellulose paper, fix in 1% phosphoric acid, and assay with a scintillation counter.
Formulation: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol
Format: Aqueous buffer solution
Storage / Stability: >12 months at -80°C. Application(s): Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s): 1. Girault, J.A., et al. Curr Opin Pharmacol. Feb,7(1):77-85 (2007)
2. Fyffe-Maricich SL., et al. J. Neurosci. Jan 19,31(3):843-50 (2011). 3. Ossum, C.G., et al. Cell Physiol Biochem. 27(2):109-20 (2011). Warning(s): Avoid freeze/thaw cycles
Scientific Category: Kinase/Serine-Threonine
Product Type: Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
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