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产品名称 ULK 1 Protein (Human)
产品货号 40099
产品价格 现货询价，电话：010-67529703
产品规格 10 µg
产品品牌 bpsbioscience
产品概述
产品详情
Species: Human
Host Species: HEK293
MW: 114 kDa
Genbank #: NM_003565
Tag(s): N-terminal FLAG-tag
a.a: 2-1050 (end)
Background:
The ULK1 protein is a serine/threonine protein kinase that plays critical role during initial stages of autophagy which is a vital response to nutrient starvation. The conserved C-terminal domain (CTD) of ULK1 controls the regulatory function and localization of the protein. Knockdown of ULK1 inhibits the autophagic response as well as inhibiting rapamycin-induced autophagy consistent with a role downstream of mTOR. ULK1 forms a complex with FIP200 and ATG13 and this complex is essential for starvation-induced autophagy. Both FIP200 and ATG13 are critical for correct localization of ULK1 to the pre-autophagosome and stability of ULK1 protein. ULK1 is phosphorylated by the mTOR pathway in a nutrient starvation-regulated manner.
Description:

Human ULK1, also known as ATG1, UNC51, and hATG1, GenBank Accession No. NM_003565, a.a. 2–1050 (end) with N-terminal Flag-tag, expressed in a HEK293 expression system. MW = 114 kDa.

Synonym(s):
ATG1, UNC51, Unc51.1, FLJ38455, Unc-51-Like Kinase, Autophagy-Related Protein 1, Human ULK1, Ulk 1, ULK1 Protein
Specific Activity:
≥85 pmol/min/µg
Formulation:
25 mM Tris-HCl, pH 7.5, 100 mM NaCl, 0.05% Tween-20, 100 µg/ml FLAG peptide, 20% glycerol and 3 mM DTT.
Format:
Aqueous buffer solution
Storage / Stability:

>6 months at -80°C.

Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):
1. Ganley I.G. et al., J. Biol. Chem. 284 (18), 12297-12305 (2009).
2. Chan E.Y. et al., Mol. Cell. Biol. 29 (1), 157-171 (2009).
3. ULK1 induces autophagy by phosphorylating Beclin-1 and activating VPS34 lipid kinase (2013)
4. AMPK and mTOR regulate autophagy through direct phosphorylation of Ulk1 (2013)
5.. mTOR inhibits autophagy by controlling ULK1 ubiquitylation, self-association and function through AMBRA1 and TRAF6 (2013)
Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Kinase/Serine-Threonine
Product Type:
Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.

Quality Assurance

Product Datasheets

40099_Lot_150806-B.pdf
产品资料