| 产品名称 | TAK1-TAB1, GST-tag - Serine-Threonine Kinases - Kinase(s) |
| 产品货号 | 40279 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | 10 µg |
| 产品品牌 | bpsbioscience |
| 产品概述 | |
| 产品详情 |
Species: Human
Host Species: Sf9 insect cells
MW: 74 kDa
Genbank #: NM_003188, NM_006116
Tag(s): N-terminal GST-tag
a.a: TAK1 (1–303), TAB1 (437–end)
Background: TAK1 is a serine/threonine protein kinase that mediates signaling by TGFβ and morphogenetic protein (BMP) (1). In response to IL-1, TAK1 forms a kinase complex with TAB1 and this complex is required for the activation of nuclear factor kappa B (Nf?B). TAK1 can also activate MAPK8/JNK and MAP2K4/MKK4 and thus play a role in the cell response to environmental stress. Tak1 is essential for thymocyte development and activation and deletion of TAK1 prevents maturation of single-positive thymocytes displaying CD4 or CD8 (2). Thymocytes lacking TAK1 fail to activate Nf?B and JNK and are prone to apoptosis upon stimulation.
Description: Recombinant fusion protein consisting of human proteins TAK1 (TGFβ-activated kinase 1), also known as MAP3K7 (Mitogen-activated protein kinase kinase kinase 7), Genbank
Accession #NM_003188, a.a. 1-303, and human TAB1 (TAK1-binding protein), Genbank Accession #NM_006116, a.a. 437-end, linked by a small peptide (DFGGGGG), and with an N-terminal GST-tag, MW = 74 kDa, expressed in Sf9 cells via a baculovirus expression system.
UniProt O43318(TAK1), Q15750 (TAB1)
Synonym(s): TAK1: MAP3K7, TGF1a, TGFβ-activated kinase 1
TAB1: MAP3K7IP1, 3'-Tab1, MGC57664, TAK1-binding protein Assay Conditions: Assay was performed in a kinase buffer containing 0.2 mM DTT using MBP substrate (0.1 mg/ml) and 100 mM ATP. Reaction was done at 30°C for 35 min. Amount of ATP transferred was calculated using ADP-Glo® reagent (Promega).
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT and 20% glycerol.
Format: Aqueous buffer solution
Storage / Stability: >6 months at –80°C
Application(s): Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s): 1.Yamaguchi, K. et al. Science 270: 2008-2011, 1995.
2.Liu, H.-H. et al. Proc. Nat. Acad. Sci. 103: 11677-11682, 2006. 3. Sakurai, H., et al. (2000). FEBS Lett. (Netherlands) 474 (2-3): 141–5. 4. Liu, H.-H., et al. (2006). Proc. Natl. Acad. Sci. USA 103: 11677-11682. Warning(s): Avoid freeze/thaw cycles
Scientific Category: Kinase/Serine-Threonine
Product Type: Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
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