| 产品名称 | SRPK2, GST-tag - Serine-Threonine Kinases - Kinase(s) |
| 产品货号 | 40278 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | 10 µg |
| 产品品牌 | bpsbioscience |
| 产品概述 | |
| 产品详情 |
Species: Human
Host Species: Sf9 insect cells
MW: 135 kDa
Genbank #: NM_182691
Tag(s): N-terminal GST-tag
a.a: full length
Background: SRPK2 is a member of the serine/arginine (SR) protein-specific kinase family that are cell cycle-regulated protein kinases which phosphorylate SR domain-containing proteins in nuclear speckles and mediate the pre-mRNA splicing events (1). SRPK2 knock down results in hypophosphorylation of the serine/arginine domain-containing human PRP28 protein thereby destabilizing PRP28 association with the tri-snRNP. RNAi-mediated depletion in HeLa cells showed that SRPK2 is essential for cell viability, and it is required for spliceosomal B complex formation (2).
Description: Recombinant human serine/arginine domain protein kinase 2 (SRPK2), full length, with N-terminal GST-tag, expressed by Sf9 insect cells via a baculovirus expression system.
Synonym(s): SFRSK2, FLJ36101
Purity: ≥75%
Formulation: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol.
Format: Aqueous buffer solution
Storage / Stability: >6 months at -80°C. Application(s): Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s): 1. Wang, H. Y. Et al; SRPK2: a differentially expressed SR protein-specific kinase involved in mediating the interaction and localization of pre-mRNA splicing factors in mammalian cells. J. Cell Biol. 140: 737-750, 1998.
2. Mathew, R. et al: Phosphorylation of human PRP28 by SRPK2 is required for integration of the U4/U6-U5 tri-snRNP into the spliceosome.Nat Struct Mol Biol. 2008 May;15(5):435-43. Warning(s): Avoid freeze/thaw cycles
Scientific Category: Kinase/Serine-Threonine
Product Type: Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
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