| 产品名称 | RSK1, GST-tag - Serine-Threonine Kinases - Kinase(s) |
| 产品货号 | 40172 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | 10 µg |
| 产品品牌 | bpsbioscience |
| 产品概述 | |
| 产品详情 |
Species: Human
Host Species: Sf9 insect cells
MW: 108 kDa
Genbank #: NM_002953
Tag(s): N-terminal GST-tag
a.a: full length
Background: RSK1 is a member of the RSK (ribosomal S6 kinase) family that are growth factor-regulated serine/threonine kinases. RSK1 contains 2 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. RSK1 encodes a predicted 735-amino acid protein containing 2 distinct consensus ATP-binding site sequences. RSK1 transcript is present in lymphocytes, skeletal muscle, liver, and adipose tissue (1). RSKs are implicated in the activation of the mitogen-activated kinase (MAPK) cascade and the stimulation of cell proliferation and differentiation (2).
Description: Recombinant human RSK1 (ribosomal S6 kinase 1), also known as MAPKAPK1A, S6K-β1, and RPS6KA1 Genbank Accession #NM_002953, full length, with an N-terminal GST-tag, MW = 108 kDa, expressed in Sf9 cells via a baculovirus expression system.
Synonym(s): RPS6KA1; HU-1; MAPKAPK1A; S6K-alpha 1
Purity: ≥90%
Specific Activity: 187 pmol/min/µg
Assay Conditions: 5 mM MOPS, 2.5 mM β-glycerophosphate, 5 mM MgCl2, 1 mM EGTA, 0.4 mM EDTA, 0.05 mM DTT, 2 mM ATP. Incubate RSK1 with 0.4 mg/ml SK6 peptide (substrate) and 50 ?M [33P]-ATP at 30°C for 15 minutes, then spot reaction on phosphocellulose paper, fix in 1% phosphoric acid, and assay with a scintillation counter. Formulation: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol.
Format: Aqueous buffer solution
Storage / Stability: >6 months at -80°C. Application(s): Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s): 1. Moller, D E. et al: Human rsk isoforms: cloning and characterization of tissue-specific expression. Am. J. Physiol. 266: C351-C359, 1994.
2. Gross, S D. et al: Induction of metaphase arrest in cleaving Xenopus embryos by the protein kinase p90(Rsk). Science 286: 1365-1367, 1999. Warning(s): Avoid freeze/thaw cycles
Scientific Category: Kinase/Serine-Threonine
Product Type: Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
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