| 产品名称 | PLK2, GST-tag - Serine-Threonine Kinases - Kinase(s) |
| 产品货号 | 40034 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | 10 µg |
| 产品品牌 | bpsbioscience |
| 产品概述 | |
| 产品详情 |
Species: Human
Host Species: Sf9 insect cells
MW: 104 kDa
Genbank #: NM_006622
Tag(s): N-terminal GST-tag
a.a: full length
Background: PLK2 or Polo-like kinase 2 is a serum-inducible kinase that is a member of the Polo family of serine/threonine protein kinases which play an essential role in normal cell division. PLK2 phosphorylates the P4.1-associated protein (CPAP), a human homologue of SAS-4, in procentriole formation during the centrosome cycle. PLK2 may also play a role as a tumor suppressor since it interacts with TSC1 protein which in turn inhibits mTOR signaling, tumor growth and chemosensitivity under hypoxic conditions. Human lung tumor cells deficient in PLK2 grow larger than control tumors, and PLK2 interacts with endogenous TSC1 protein in these tumors.
Description: Human Plk2 also known as SNK, polo-like kinase 2, GenBank Accession No. NM_006622, a.a. full length, with N-terminal GST-tag, expressed in a baculovirus infected Sf9 cell expression system. MW=104 kDa.
UniProt Q9NYY3
Synonym(s): SNK, polo-like kinase 2
Supplied As: Aqueous buffer solution.
Specific Activity: ≥1.6 pmole/min/μg
Assay Conditions: 40 mM Tris-HCl pH 7.4, 20 mM MgCl2, 0.1 mg/mL BSA and 1 mM DTT using 0.5 mg/ml Casein substrate and 10 μM ATP. Reaction was done at 30°C for 45 min. Amount of ATP transferred was calculated using Kinase-Glo reagent from Promega.
Formulation: 40 mM Tris pH 8, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween, 3 mM DTT, and 20% glycerol.
Storage / Stability: At least 6 months at -80°C.
Application(s): Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s): 1. Syed, N. et al., Blood. 2006 Jan 1,107(1):250-6.
2. Eckerdt, F. et al., Oncogene. 2005 Jan 10,24(2):267-76. Warning(s): Avoid freeze/thaw cycles
Scientific Category: Kinase/Serine-Threonine
Product Type: Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
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