| 产品名称 | PLK1, His-tag - Serine-Threonine Kinases - Kinase(s) |
| 产品货号 | 40033 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | 10 µg |
| 产品品牌 | bpsbioscience |
| 产品概述 | |
| 产品详情 |
Species: Human
Host Species: Sf9 insect cells
MW: 69 kDa
Genbank #: NM_005030
Tag(s): C-terminal His-tag
a.a: full length
Background: PLK1 is a member of the Polo-Like Kinase family that localize to centrosomes or spindle pole bodies and undergo dramatic subcellular relocation during the cell cycle. Deregulated activities of PLKs often result in abnormalities in centrosome duplication, maturation, and/or microtubule dynamics. PLKs also regulate the function of the Golgi complex. Deregulated expression of human PLK1 is strongly correlated with the development of many types of malignancies, and ectopic expression of PLK1 dominant negative protein leads to rapid cell death.
Description: Human Plk1 also known as STPK13 and polo-like kinase 1, GenBank Accession No. NM_005030, a.a. full length with C-terminal His-tag, expressed in a Baculovirus infected Sf9 cell expression system. MW=69 kDa.
UniProt P53350
Synonym(s): STPK13, polo-like kinase 1
Supplied As: Aqueous buffer solution.
Specific Activity: ≥14 pmol/min/µg
Assay Conditions: 40 mM Tris-HCl pH 7.4, 20 mM MgCl2, 0.1 mg/mL BSA and 1 mM DTT using 1 mg/ml Casein substrate and 20 μM ATP. Reaction was done at 30°C for 45 min. Amount of ATP transferred was calculated using Kinase-Glo reagent from Promega.
Formulation: 40 mM Tris-HCl pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol and 250 mM Imidazole.
Format: Aqueous buffer solution
Storage / Stability: At least 6 months at -80°C.
Application(s): Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s): 1. Fabbro, M. et al., Dev. Cell 9 (4), 477-488. (2005).
2. Liu, X., et al., Mol. Cell. Biol. 25, 4993-5010 (2005). Warning(s): Avoid freeze/thaw cycles
Scientific Category: Kinase/Serine-Threonine
Product Type: Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
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