| 产品名称 | PKCα (PKCalpha), GST-tag - Serine-Threonine Kinases - Kinase(s) |
| 产品货号 | 40157 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | 10 µg |
| 产品品牌 | bpsbioscience |
| 产品概述 | |
| 产品详情 |
Species: Human
Host Species: Sf9 insect cells
MW: 102 kDa
Genbank #: NM_002737
Tag(s): N-terminal GST-tag
a.a: full length
Background: PKCα (PKC alpha) is a member of the protein kinase C (PKC) family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC-alpha has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control(1). PKCα has been assigned to the chromosome region 17q22-q23.2 and has been identified as a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes (2).
Description: Recombinant human PKCα (PKCalpha), full length, GenBank Accession No. NM_002737, with N-terminal GST-tag, expressed in Sf9 insect cells via a baculovirus expression system. MW= 102 kDa.
Synonym(s): AAG6, PKCA, PRKCA, PRKACA, MGC129900, MGC129901, PKCα protein kinase c alpha
Purity: ≥80%
Specific Activity: 320 pmol/min/µg
Assay Conditions: Assay was done in Kinase buffer containing 0.1 mM CaCl2 and lipid activator (1 mg/ml Phosphatidyl Serine and 0.02 mg/ml Diacylglycerol in 0.3% TritonX-100) using Histone H1 (0.1 mg/ml) and 20 μM ATP. Reaction was done at 30°C for 40 min. Amount of ATP transferred was calculated using Kinase-Glo reagent (Promega)
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween20, 3 mM DTT, 20% glycerol, 1.6 mM Glutathione
Format: Aqueous buffer solution
Storage / Stability: At least 6 months at –80°C. Avoid freeze/thaw cycles. Protein may be diluted to ≥ 100 μg/ml in PBS + glycerol and stored at -80°C.
Application(s): Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s): 1. Coussens, L. et al. Science 233: 859-866, 1986.
2. Braz, J. C. et al. Nature Med. 10: 248-254, 2004. Warning(s): Avoid freeze/thaw cycles
Scientific Category: Kinase/Serine-Threonine
Product Type: Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
|
| 产品资料 |
