| 产品名称 | PIM1, Active, GST-tag - Serine-Threonine Kinases - Kinase(s) |
| 产品货号 | 41107 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | 10 µg |
| 产品品牌 | bpsbioscience |
| 产品概述 | |
| 产品详情 |
Species: Human
Host Species: Sf9 Insect Cells
MW: 62 kDa
Genbank #: NM_002648
Tag(s): N-terminal GST tag
a.a: full-length
Background: PIM1 is a proto-oncogene that belongs to a family of serine/threonine protein kinases that are highly conserved through evolution in multicellular organisms. Originally identified from Moloney murine leukemia virus induced T-cell lymphomas in mice, PIM1 is involved in the control of cytokine-mediated cell proliferation, differentiation and survival of lymphoid and myeloid cells as well as others (1). Expression of PIM1 can be stimulated by a variety of growth factors and is regulated at four different levels: transcriptional, post-transcriptional, translational and post-translational (2). Expression of PIM1 is mediated through activation of the JAK/STAT pathway.
Description: Recombinant human
serine/threonine-protein kinase PIM1
(PIM1), GenBank Accession No.
NM_002648, full-length with an N-terminal
GST tag and expressed in a baculovirusinfected
Sf9 insect cell expression system.
MW = 62 kDa.
UniProt P11309
Synonym(s): serine/threonine-protein kinase PIM1
Specific Activity: 316 pmol/min/µg
Assay Conditions: Varying amounts of
enzyme were incubated in 5 mM MOPS,
pH 7.2, 2.5 mM -glycerophosphate, 4
mM MgCl2, 2.5 mM MnCl2, 1 mM EGTA,
0.4 mM EDTA, 0.01 mM DTT, 0.05 mM
ATP, 10 μg of S6K synthetic peptide
substrate, and 32 μCi of [33P]-ATP was
incubated at 30°C for 15 minutes. Then
spot reaction on phosphocellulose paper,
fix in 1% phosphoric acid, and assay with
a scintillation counter.
Formulation: 50 mM Tris-HCl, pH 7.5, 150
mM NaCl, 10 mM glutathione, 0.1 mM EDTA,
0.1 mM PMSF, 0.25 mM DTT, 25% glycerol
Format: Aqueous buffer solution.
Storage / Stability: At least 6 months at –80°C.
Application(s): Useful for studying enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s): 1. Meeker, T.C., et al., J. Cell. Biochem. 1987; 35(2): 105-112.
2. Friedmann, M. et al., Arch. Biochem Biophys. 1992; 298(2): 594-601. Warning(s): Avoid
freeze/thaw cycles. Storing diluted enzyme is
not recommended, if necessary, use carrier
protein (BSA 0.1 – 0.5%).
Scientific Category: Kinase - Serine/Threonine
Product Type: Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
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