| 产品名称 | PAK5, His-tag - Serine-Threonine Kinases - Kinase(s) |
| 产品货号 | 40077 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | 10 µg |
| 产品品牌 | bpsbioscience |
| 产品概述 | |
| 产品详情 |
Species: Human
Host Species: E. coli
MW: 33.9 kDa
Genbank #: NM_177990
Tag(s): N-terminal His-tag
a.a: 425–719
Background: PAK5 is a protein kinase of the STE20 family that is predominantly expressed in brain. It is capable of promoting neurite outgrowth, and thus may play a role in neurite development. Associated with microtubule networks and induces microtubule stabilization. Its subcellular localization is tightly regulated during cell cycle progression.
Description: Human PAK5 (GenBank Accession No. NM_177990), a.a. 425-719, MW=33.9 kDa, expressed in E. coli.
Purity: ≥95%
Specific Activity: 4.2 nmoles/min/μg
Assay Conditions: Analysis of enzymatic activity was performed according to Zlyte assay protocol (Invitrogen). PAK5 was incubated in a buffer containing 50 mM HEPES (pH 7.5), 10 mM MgCl2, 1 mM EGTA, 200 μM ATP, 0.01% Brij-35, and 2 μM substrate (SER/THR 20, Invitrogen) at room temperature for 1 hour. Developer solution was added to reaction for 1 hour at RT. Fluorescence was detected using λexc=460±40 nm and λem=528±20 nm
Formulation: 25 mM Tris-HCl, pH 7.0, 50 mM NaCl, 10% glycerol, 5 mM DTT
Format: Aqueous buffer solution
Storage / Stability: >6 months at –80°C
Application(s): Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s): 1. Li,Y., et al., PLoS ONE 3 (7), E2707 (2008).
2. Ewing,R.M., et al., Mol. Syst. Biol. 3, 89 (2007). Warning(s): Avoid freeze/thaw cycles
Scientific Category: Kinase/Serine-Threonine
Product Type: Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
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