| 产品名称 | PAK2 - Serine-Threonine Kinases - Kinase(s) |
| 产品货号 | 40073 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | 10 µg |
| 产品品牌 | bpsbioscience |
| 产品概述 | |
| 产品详情 |
Species: Human
Host Species: E. coli
MW: 33.5 kDa
Genbank #: NM_002577
Tag(s): none
a.a: 227–524
Background: PAK proteins is a family of serine/threonine kinases that serve as targets for the small GTP binding proteins, CDC42 and RAC1, and have been implicated in a wide range of biological activities. The p21 activated kinases (PAK) are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. PAK2 is activated by proteolytic cleavage during caspase-mediated apoptosis, and may play a role in regulating the apoptotic events in the dying cell. The CDC42 and RAC1 induce autophosphorylation of PAK2, which stimulates sustained phosphorylation of other substrates.
Description: Human PAK2 (GenBank Accession No. NM_002577), a.a. 227-524, MW=33.5 kDa, expressed in E. coli.
Purity: ≥95%
Specific Activity: 86 nmoles/min/μg
Assay Conditions: Analysis of enzymatic activity was performed according to Zlyte assay protocol (Invitrogen). PAK2 was incubated in a buffer containing 50 mM HEPES (pH 7.5), 10 mM MgCl2, 1 mM EGTA, 200 μM ATP, 0.01% Brij-35, and 2 μM substrate (SER/THR 20, Invitrogen) at room temperature for 1 hour. Developer solution was added to reaction for 1 hour at RT. Fluorescence was detected using λexc=460±40 nm and λem=528±20 nm
Formulation: 25 mM Tris-HCl, pH 8, 150 mM NaCl, 10% glycerol, 5 mM DTT
Format: Aqueous buffer solution
Storage / Stability: >6 months at –80°C.
Application(s): Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s): 1. Coniglio,S.J., et al., Mol. Cell. Biol. 28 (12), 4162-4172 (2008).
2. Koh,W., et al., J. Cell. Sci. 121 (PT 7), 989- 1001 (2008). Warning(s): Avoid freeze/thaw cycles
Scientific Category: Kinase/Serine-Threonine
Product Type: Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
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