| 产品名称 | p70S6Kb, GST-tag - Serine-Threonine Kinases - Kinase(s) |
| 产品货号 | 40146 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | 10 µg |
| 产品品牌 | bpsbioscience |
| 产品概述 | |
| 产品详情 |
Species: Human
Host Species: Sf9 insect cells
MW: 85 kDa
Genbank #: BC000094
Tag(s): N-terminal GST-tag
a.a: full length
Background: p70S6Kb is a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases and is activated by mitogenic stimuli, including growth factors, cytokines, and phorbol esters. p70S6Kb contains 2 nonidentical kinase catalytic domains and phosphorylates the S6 ribosomal protein and eucaryotic translation initiation factor 4B. Phosphorylation of S6 leads to an increase in protein synthesis and cell proliferation. PI3 kinase pathway and mTOR are involved in the activation of p70S6Kb but other pathways can also activate this target protein.
Description: Recombinant human
p70S6Kb, also known as RPS6KB2,
STK14B, KLS, P70-beta, or SRK,
GenBank Accession No. BC_000094, fulllength
with an N-terminal GST tag and
expressed in a baculovirus-infected Sf9
insect cell expression system. MW = 85
kDa.
Synonym(s): RPS6KB2, STK14B, KLS, P70-beta, P70-beta-1, P70-beta-2, S6K2, p70(S6K)-beta, S6K-beta2, SRK
Purity: ≥90%
Specific Activity: 107 pmol/min/µg
Assay Conditions: 5 mM MOPS, 2.5 mM β-glycerophosphate, 5 mM MgCl2, 1 mM EGTA, 0.4 mM EDTA, 0.05 mM DTT, 2 mM ATP. Incubate p70S6K with 0.2 mg/ml RSK-sub peptide substrate and 50 ?M [33P]-ATP at 30°C for 15 minutes, then spot reaction on phosphocellulose paper, fix in 1% phosphoric acid, and assay with a scintillation counter.
Formulation: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol.
Format: Aqueous buffer solution
Storage / Stability: At least 6 months at -80°C.
Application(s): Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s): 1. Gout, I. et al: Molecular cloning and characterization of a novel p70 S6 kinase, p70 S6 kinase beta containing a proline-rich region. J. Biol. Chem. 273: 30061-30064, 1998.
2. Saitoh, M. et al: Cloning and characterization of p70(S6K-beta) defines a novel family of p70 S6 kinases. Biochem. Biophys. Res. Commun. 253: 470-476, 1998. Warning(s): Avoid freeze/thaw cycles
Scientific Category: Kinase/Serine-Threonine
Product Type: Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
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