Human NEK6 (GenBank Accession No. NM_014397), a.a. 1-313 with N-terminal His-tag, MW=40.1 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

NEK6, NIMA-related kinase 6, Never In Mitosis-Related Kinase 6

70 U/mg

One unit is defined as the amount of enzyme that will phosphorylate 1 nmol NEK6 substrate (FLAKSFGSPNRAYKK) per minute at pH 7.4 and 30°C.

50 mM HEPES, pH 7.4, 3 mM MgCl₂, 3 mM MnCl₂, 1 mM DTT, 3 µM Na orthovanadate, 0.1 mM ATP, 4 µM NEK6 substrate, and 0.4 µg/ml NEK6.

25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol, and 3 mM DTT.

Aqueous buffer solution

>6 months at -80°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Yin, M.J. et al., J. Biol. Chem. 278 (52), 52454-52460 (2003)
2. Belham, C. et al., J. Biol. Chem. 278 (37), 34897-34909 (2003)

Avoid freeze/thaw cycles

Kinase/Serine-Threonine

Recombinant Protein