Human serine/threonineprotein kinase/endoribonuclease IRE1 (IRE1), a.a. 469-977 with a N-terminal His-tag, TEV sequence, and AviTagTM. MW = 62 kDa, expressed in baculovirus-infected Sf9 insect cell expression system.

O75460

Serine/threonine-protein kinase/endoribonuclease IRE1; ERN1; Endoplasmic reticulum-to-nucleus signaling 1

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, 300 mM Imidazole

Aqueous buffer solution

At least 6 months at -80°C

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Urano, F., et al., Science. 2000; 287(5453): 664-666.
2. Calfon, M., et al., Nature. 2002; 415: 92- 96.

The aggregation state of this product has been confirmed to be <10%.

Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use a carrier protein (BSA 0.1 – 0.5%).

Kinase

Serine/threonine