Human GSK3β GenBank Accession No. NM_002093), full length, with N-terminal GST-tag, MW=73 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

P49841

GSK3b, glycogen synthase kinase 3 beta, GSK3beta

391 pmol/min/µg

Enzyme reactions include 50 μL volumes of 1x Kinase buffer (40 mM Tris pH 7.4, 20 mM MgCl₂, 0.1 mg/mL BSA) along with 20 μM ATP, 0.2 mg/mL GSKtide substrate, 3mM DTT, and GSTGSK3ß. After 45 min incubation at 30°C, 50 μL of the Kinase-Glo reagent solution was added and luminescence from remaining ATP was measured.

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 16 mM glutathione

Aqueous buffer solution

>6 months at -80°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Sutherland C. et al. Biochem J. 1993 Nov 15;296 (Pt 1):15-9.
2. Sperber BR et al. Neurosci Lett. 1995 Sep 8;197(2):149-53.

Application Reference(s):
1. Glycogen synthase kinase-3β inhibition in the medial prefrontal cortex mediates paradoxical amphetamine action in a mouse model of ADHD (2015)
2. Induction of Gsk3β-β-TrCP interaction is required for late phase stabilization of β-catenin in canonical Wnt signaling (2014)
3. Leucine-rich repeat kinase 2 regulates tau phosphorylation through direct activation of glycogen synthase kinase-3β (2014)

Avoid freeze/thaw cycles

Kinase/Serine-Threonine

Recombinant Protein