| 产品名称 | GRK6, GST-tag - Serine-Threonine Kinases - Kinase(s) |
| 产品货号 | 40066 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | 10 µg |
| 产品品牌 | bpsbioscience |
| 产品概述 | |
| 产品详情 |
Species: Human
Host Species: Sf9 insect cells
MW: 93 kDa
Genbank #: BC017272
Tag(s): N-terminal GST-tag
a.a: full length
Background: GRK6 is a Ser/Thr protein kinase and a member of the G protein-coupled receptor kinase subfamily. GRK6 is present in all tissues with strongest expression in placenta and skeletal muscle. GRK6 can phosphorylate proteins such as rhodopsin, ADRB2 and the leukotriene B4 receptor BLT1 (1). GRK6 phosphorylates G protein-coupled dopamine receptors, thereby regulating their activity and mediating desensitization of the receptors. GRK6 knockout mice showed supersensitivity to the locomotor-stimulating effects of psychostimulants, including cocaine and amphetamine (2).
Description: Human GRK6, also known as GPRK6 or FLJ3213 (GenBank Accession No. BC_017272), full length, with N-terminal GST tag, MW ~93 kDa, expressed in Sf9 insect cells via a
baculovirus expression system.
Synonym(s): GPRK6; FLJ32135
Purity: ≥80%
Specific Activity: 11 pmol/min/µg
Assay Conditions: 5 mM MOPS, 2.5 mM β-glycerophosphate, 5 mM MgCl2, 1 mM EGTA, 0.4 mM EDTA, 0.05 mM DTT, 2 mM ATP. Incubate GRK6 with 0.25 mg/ml PLKtide(substrate) and 50 ?M [33P]-ATP at 30°C for 15 minutes, then spot reaction on phosphocellulose paper, fix in 1% phosphoric acid, and assay with a scintillation counter.
Formulation: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol
Format: Aqueous buffer solution
Storage / Stability: >6 months at -80°C. Application(s): Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s): 1. Gaudreau, R. et al: Threonine 308 within a putative casein kinase 2 site of the cytoplasmic tail of leukotriene B(4) receptor (BLT1) is crucial for ligand-induced, G-protein-coupled receptor-specific kinase 6-mediated desensitization. J. Biol. Chem. 277: 31567-31576, 2002.
2. Gainetdinov, R R. et al: Dopaminergic supersensitivity in G protein-coupled receptor kinase 6-deficient mice. Neuron 38: 291-303, 2003. Warning(s): Avoid freeze/thaw cycles
Scientific Category: Kinase/Serine-Threonine
Product Type: Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
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