Human endoplasmic reticulumto- nucleus signaling 1 (ERN1), also known as inositol-requiring protein 1 (IRE1), GenBank Accession No. NM_001433, a.a. 468-977 (end) MW = 87 kDa, expressed in a baculovirus-infected Sf9 insect cell expression system.

O75460

inositol-requiring protein 1 (IRE1), IRE

55 pmol/min/μg

10 μl of varying amounts of ERN1 was mixed with 5 μl of 1 mg/ml myelin basic protein substrate and 5 μl of H2O. Reaction was initiated by adding 5 μl of 250 μM [33P]-ATP and incubating for 15 min at 30°C followed by spotting the reaction on phosphocellulose paper, fixing in 1% phosphoric acid, and assaying with a scintillation counter.

50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutatione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.

Aqueous buffer solution

>6 months at -80°C

Useful for studying enzyme kinetics and screening small molecule inhibitors.

1. Niwa, M., et al., Cell. 1999; 99: 691-702.

2. Iwawaki, T. et al., Proc. Nat. Acad. Sci. 2009; 106: 16657-16662.

Avoid freeze/thaw cycles. Storing diluted protein is not recommended, if necessary, use carrier protein (BSA 0.1 – 0.5%).

Kinase/Serine Threonine

Recombinant Protein