Human eIF4e (eukaryotic translation initiation factor 4E) (GenBank Accession No. NM_001968), a.a. 2-216 with N-terminal GST-tag, MW= 51 kDa, expressed in an E. coli expression system.

P06730

EIF4E1, Eukaryotic Translation Initiation Factor 4E, mRNA Cap-Binding Protein, EIF-4E

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 16 mM glutathione

Aqueous buffer solution

>12 months at -80°C.

Suitable substrate for MNK1 kinase and other kinase assays.

1. Shih,J.W. et al., Oncogene 27(5), 700-714 (2008).
2. Salehi Z., Mashayekhi F. and Shahosseini F. Cell Biol. Int. 31(11), 1400-1404 (2007).

Application Reference:
MNK1-induced eIF-4E phosphorylation in myeloma cells: a pathway mediating IL-6-induced expansion and expression of genes involved in metabolic and proteotoxic responses (2014)

Avoid freeze/thaw cycles

Kinase/Substrate

Recombinant Protein