Human Checkpoint kinase 2 (GenBank Accession No. NM_007194), full length, with N-terminal His-tag. MW=91 kDa. Expressed in a Baculovirus infected Sf9 cell expression system.

CHK2, CHEK2, checkpoint kinase 2 , CDS1, RAD53

6.9 pmoles/min/µg

CHEK2 was incubated with a substrate for 1h at RT in kinase buffer supplemented with ATP. Developer solution was added to reaction and reaction was stopped after 1 hour of incubation at RT.

50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 40% glycerol and 3 mM DTT.

Aqueous buffer solution

>12 months at -80°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Reinhardt HC and Yaffe MB. Curr Opin Cell Biol. 2009 Apr;21(2):245-55.
2. Stracker TH and Petrini JH. Cell Cycle. 2008 Dec,7(23):3618-21.

Avoid freeze/thaw cycles

Kinase/Serine-Threonine

Recombinant Protein