Human cyclin-dependent kinase 9 (CDK9), also known as C-2K and cell division protein kinase 9, complexed with human cyclin T1 (CCNT1, CycT1), GenBank Accession Nos. NM_001261 (CDK9) and NM_001240 (CyclinT1), a.a. 2- 372(end) (CDK9) and a.a. 2-726(end) (CyclinT1) each with N-terminal GST-tags, co-expressed in a baculovirus-infected Sf9 cell expression system. MW = 69 kDa (CDK9) and 107 kDa (CyclinT1).
| 产品名称 | CDK9/CyclinT1, GST-tag - Serine-Threonine Kinases - Kinase(s) |
| 产品货号 | 40307 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | 10 µg |
| 产品品牌 | bpsbioscience |
| 产品概述 | |
| 产品详情 |
Species: Human
Host Species: Sf9 cell
MW: CDK9: 69 kDa; CyclinT1: 107 kDa
Genbank #: CDK9: NM_001261; CyclinT1: NM_001240
Tag(s): N-terminal GST-tag
a.a: CDK9: 2- 372(end); CyclinT1: 2-726(end)
Description: UniProt CDK9: P50750; CyclinT1: O60563
Synonym(s): cyclin-dependent kinase 9, C-2K, cell division protein kinase 9, and CCNT1 , CycT1
Specific Activity: 26 pmol/min/μg
Assay Conditions: Assay was done in a
Kinase buffer using PDKtide (0.1 mg/ml) as
a substrate with 10 M ATP at 30°C for 45
min. The amount of ATP transferred from
autophosphorylation as well as substrate
phosphorylation was calculated using
Kinase-Glo reagent (Promega).
Formulation: 40 mM Tris-HCl, pH 8.0,
110 mM NaCl, 2.2 mM KCl, 0.04%
Tween-20, 3 mM DTT, 20% glycerol, and
1.6 mM glutathione
Format: Aqueous buffer solution
Storage / Stability: >6 months at -80°C
Application(s): Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s): 1. Yang, Z., et al., Nat. 2001; 414: 317-322. 2. Baumli, S., et al., EMBO J. 2008; 27(13): 1907-1918. Warning(s): Avoid freeze/thaw cycles. Storing diluted
enzyme is not recommended, if
necessary, use carrier protein (BSA 0.1
– 0.5%).
Scientific Category: Kinase - Serine/Threonine
Product Type: Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
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