| 产品名称 | CDK6/CyclinD1, His-tag, GST-tag - Serine-Threonine Kinases - Kinase(s) |
| 产品货号 | 40097 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | 10 µg |
| 产品品牌 | bpsbioscience |
| 产品概述 | |
| 产品详情 |
Species: Human
Host Species: Sf9 insect cells
MW: 40 kDa (CDK6), 61 kDa (Cyclin D1)
Genbank #: NM_001259 /NM_053056
Tag(s): N-terminal His-tag (CDK6), N-terminal GST-tag (CyclinD1)
a.a: full length
Background: CDK6 is a member of the cyclin-dependent family of protein kinases that are important regulators of cell cycle progression. CDK6 activity is regulated by the D-type cyclins and members of the INK4 family of CDK inhibitors (1). The CDK6 kinase activity is detected in mid-G1 phase of the cell cycle and is responsible for the phosphorylation and regulation of the activity of tumor suppressor protein Rb. Although CDK6 and CDK4 can both phosphorylate multiple residues in the Rb protein, they do so with different residue selectivity in vitro; CDK6 phosphorylates Thr821 while CDK4 phosphorylates Thr826 on Rb protein (2).
Description: Complex of recombinant human CDK6, full length with N-terminal His-tag, and Cyclin D1, full length with N-terminal GST-tag (GenBank Accession Nos. NM_001259, and
NM_053056 respectively) co-expressed in Sf9 insect cells via a baculovirus expression system.
Synonym(s): Cyclin-dependent kinase 6, CDK6: PLSTIRE, MGC59692
Cyclin D1: BCL1, PRAD1, U21B31, D11S287E Purity: ≥80%
Specific Activity: 8 pmol/min/µg
Formulation: 50 mM sodium phosphate pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.2 mM DTT, 25% glycerol
Format: Aqueous buffer solution
Storage / Stability: >6 months at -80°C. Application(s): Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s): 1. Meyerson, M. et al: Identification of G1 kinase activity for cdk6, a novel cyclin D partner. Molec. Cell. Biol. 14: 2077-2086, 1994.
2. Takaki, T. et al: Preferences for phosphorylation sites in the retinoblastoma protein of D-type cyclin-dependent kinases, Cdk4 and Cdk6, in vitro. J Biochem. 2005 Mar;137(3):381-6. Warning(s): Avoid freeze/thaw cycles
Scientific Category: Kinase/Serine-Threonine
Product Type: Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
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