产品名称 CDK4/CyclinD3, GST-His-Tag - Serine-Threonine Kinases - Kinase(s)
产品货号 40104
产品价格 现货询价,电话:010-67529703
产品规格 10 µg
产品品牌 bpsbioscience
产品概述
产品详情
Species: Human
Host Species: Baculovirus-infected Sf9 Cell
MW: 64 kDa (CDK4), 59 kDa (Cyclin D3)
Genbank #: NM_000075/NM_001760
Tag(s): N-terminal GST-His-Tag/N-terminal GST-tag
a.a: 1-303(end)/2-292(end)
Description:
Complex of recombinant human CDK4, a.a. 1-303 (end), with N-Terminal GST-His-Tag, GenBank Accession No. NM_000075, and Cyclin D3, a.a 2-292(end), with N-terminal GST-tag, GenBank Accession No. NM_001760. Co-expressed in a Baculovirus-infected Sf9 cell expression system. CDK4 MW=64 kDa, Cyclin D3 MW=59 kDa.
Synonym(s):
Cyclin-Dependent Kinase 4, CDK4: CMM3; PSK-J3; MGC14458
Cyclin D3: CCND3
Specific Activity:
10 pmole/min/ug
Assay Conditions:
Assay was done in a Kinase buffer using Rb (0.02 mg/ml) as a substrate with 10 μM ATP at 30°C for 60 min. The amount of ATP transferred was calculated using Kinase-Glo reagent (Promega).
Formulation:
40mM Tris-HCl pH 8.0, 110mM NaCl, 2.2mM KCl, 20% Glycerol and 3mM DTT and 2 mM Glutathione
Format:
Aqueous buffer solution.
Storage / Stability:

At least 6 months at -80°C.

Application(s):
Useful for three-dimensional structure studies.
Reference(s):

1. Bullrich, F. et al., Cancer Res. (1995); 55:1199-1205.

2. Ye, X. et al., Proc. Nat. Acad. Sci.. (2001); 98:1682-1686.

Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Kinase/Serine-Threonine
Product Type:
Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.

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Product Datasheets

40104.pdf
产品资料