产品名称 | CDK4/CyclinD3, GST-His-Tag - Serine-Threonine Kinases - Kinase(s) |
产品货号 | 40104 |
产品价格 | 现货询价,电话:010-67529703 |
产品规格 | 10 µg |
产品品牌 | bpsbioscience |
产品概述 | |
产品详情 |
Species: Human
Host Species: Baculovirus-infected Sf9 Cell
MW: 64 kDa (CDK4), 59 kDa (Cyclin D3)
Genbank #: NM_000075/NM_001760
Tag(s): N-terminal GST-His-Tag/N-terminal GST-tag
a.a: 1-303(end)/2-292(end)
Description: Complex of recombinant human CDK4, a.a. 1-303 (end), with N-Terminal GST-His-Tag, GenBank Accession No. NM_000075, and Cyclin D3, a.a 2-292(end), with N-terminal GST-tag, GenBank Accession No. NM_001760. Co-expressed in a Baculovirus-infected Sf9 cell expression system. CDK4 MW=64 kDa, Cyclin D3 MW=59 kDa.
Synonym(s): Cyclin-Dependent Kinase 4, CDK4: CMM3; PSK-J3; MGC14458
Cyclin D3: CCND3 Specific Activity: 10 pmole/min/ug
Assay Conditions: Assay was done in a Kinase buffer using Rb (0.02 mg/ml) as a substrate with 10 μM ATP at 30°C for 60 min. The amount of ATP transferred was calculated using Kinase-Glo reagent (Promega).
Formulation: 40mM Tris-HCl pH 8.0, 110mM NaCl, 2.2mM KCl, 20% Glycerol and 3mM DTT and 2 mM Glutathione
Format: Aqueous buffer solution.
Storage / Stability: At least 6 months at -80°C. Application(s): Useful for three-dimensional structure studies.
Reference(s): 1. Bullrich, F. et al., Cancer Res. (1995); 55:1199-1205. 2. Ye, X. et al., Proc. Nat. Acad. Sci.. (2001); 98:1682-1686. Warning(s): Avoid freeze/thaw cycles.
Scientific Category: Kinase/Serine-Threonine
Product Type: Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
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产品资料 |