| 产品名称 | CAMK2α, GST-tag - Serine-Threonine Kinases - Kinase(s) |
| 产品货号 | 40038 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | 10 µg |
| 产品品牌 | bpsbioscience |
| 产品概述 | |
| 产品详情 |
Species: Human
Host Species: Sf9 insect cells
MW: 74 kDa
Genbank #: NM_171825
Tag(s): N-terminal GST-tag
a.a: full length
Background: CAMK2α is a ser/thr protein kinase that is a member of the Ca2+/calmodulin-dependent protein kinase family. CAMK2α is abundant in the brain as a major constituent of the postsynaptic density and is required for hippocampal long-term potentiation (LTP) and spatial learning. In addition to its Ca2+/calmodulin-dependent activity, CAMK2α can undergo autophosphorylation, resulting in Ca2+/calmodulin-independent activity. The protein level of CAMK2α fluctuates during neuronal activity in cultured rat pup hippocampal neurons. The levels of CAMK2α increased with heightened neuronal activity (2).
Description: Recombinant human calcium/calmodulin-dependent protein kinase II alpha (CaMK2α), GenBank Accession #NM_171825, full length, with an N-terminal GST-tag, MW = 74 kDa, expressed in Sf9 cells via a baculovirus expression system.
Synonym(s): CAMKA; KIAA0968
Purity: ≥75%
Specific Activity: 294 pmol/min/µg
Assay Conditions: 5 mM MOPS, 2.5 mM β-glycerophosphate, 5 mM MgCl2, 1 mM EGTA, 0.4 mM EDTA, 0.05 mM DTT, 50 ?g/ml BSA, 2 mM ATP. 0.5 mM CaCl2, 0.03 mg/ml calmodulin. Incubate CaMK2α with 0.3 mg/ml autocamtide-2 peptide as substrate and 50 ?M [33P]-ATP at 30°C for 15 minutes, then spot reaction on phosphocellulose paper, fix in 1% phosphoric acid, and assay with a scintillation counter.
Formulation: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol.
Format: Aqueous buffer solution
Storage / Stability: >6 months at -80°C. Application(s): Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s): 1. Silva, A J. et a . Science 257: 206-211, 1992.
2. Thiagarajan, T C. et al . Neuron 36: 1103-1114, 2002. 3. Salas, M.A., et al. J. Mol. Cell Cardiol. (2010) 48(6):1298-130. 4. Chen, Y., et al. Neurochem Res. (2008) 33(10):2092-8. Warning(s): Avoid freeze/thaw cycles
Scientific Category: Kinase/Serine-Threonine
Product Type: Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
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