Human BRAF (b-RAF) 9GenBank Accession #NM_004333), a.a. 417-766 (end) with N-terminal GST-tag, MW = 66 kDa, expressed in Sf9 cells using a Baculovirus expression system.

P15056

B-RAF1, BRAF, RAFB1

44 pmol/min/µg

Reaction was performed in a kinase buffer (40 mM Tris, 10 mM MgCl₂, 0.1 mg/ml BSA)containing 2 μg inactive MEK1 (BPS #40075), 5 μM ATP, and 0.1 – 1.6 ng/μl BRAF for 60 minutes at room temperature. The amount of ATP transferred was measured by BPS ATP quantification reagents.

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 16 mM glutathione

Aqueous buffer solution

>6 months at -80°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Sithanandam G., et al. (1990). Oncogene. 5(12): 1775–1780.
2. Shepherd C., et al. (2010). Curr. Oncol. Rep. 12(3):146-152.

Avoid freeze/thaw cycles

Kinase/Serine-Threonine

Recombinant Protein