| 产品名称 | BRAF (V600E), GST-tag - Serine-Threonine Kinases - Kinase(s) |
| 产品货号 | 40533 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | 10 µg |
| 产品品牌 | bpsbioscience |
| 产品概述 | |
| 产品详情 |
Species: Human
Host Species: Sf9 insect cells
MW: 69 kDa
Genbank #: NM_004333
Tag(s): N-terminal GST-tag
a.a: 417–end
Background: BRAF is a member of the RAF family that is activated by members of the Ras family upon growth factor-induced stimulation. Active Ras can induce heterodimerization of cRaf and BRAF and this may explain the observed cooperativity of cRaf and BRaf in cells responding to growth factor signals (1). Activating mutations in the BRAF gene are present in a large percentage of human malignant melanomas and in a proportion of colon cancers. The vast majority of these mutations result in a valine to glutamic acid change at residue 600 within the activation segment of B-RAF (2).
Description: Human v-raf murine sarcoma viral oncogene homolog B1 (BRAF), GenBank Accession No. NM_004333, a.a. 417-end with Val-to-Glu mutation on a.a. 600* and N-terminal GST-tag, MW=69 kDa, expressed in Sf9 insect cells via a baculovirus expression system.*This mutation is often cited as V600E.
UniProt P15056
Synonym(s): BRAF1, RAFB1, B-raf 1, MGC126806, MGC138284, BRAF (V600E)
Specific Activity: 150 pmol/min/μg Assay Conditions: Assay was performed in a kinase buffer containing 0.1 mg/ml inactive MEK1 substrate (BPS Cat# 40075) and 10 μM ATP. Reaction was done at 30°C for 30 minutes. ATP transferred was calculated using Kinase-Glo® (Promega).
Formulation: 40 mM Tris, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT and 20% glycerol.
Format: Aqueous buffer solution
Storage / Stability: >6 months at –80°C
Application(s): Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s): 1. Weber, C.K., et al., Cancer Res. 2001 May 1, 61(9): 3595-8.
2. Mercer, K.E., et al., Biochim Biophys Acta. 2003 Jun 5, 653(1): 25-40. Warning(s): Avoid freeze/thaw cycles
Scientific Category: Kinase/Serine-Threonine
Product Type: Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
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