Human Aurora kinase B (GenBank Accession No. NM_004217), a.a. 2-344, with N-terminal GST-tag and C-terminal His-tag, MW=66 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Aurora Kinase B, STK12, STK5, AIM-1, IPL1, ARK2, AURB

6.2 U/µg.

One unit is defined as the amount of enzyme that will phosphorylate 1 pmol of Ser/Thr 1 peptide substrate per minute at pH 7.4 and 30°C.

Assay buffer: 50 mM HEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3 uM Na-orthovanadate, 0.5 mM ATP, 4 uM aurora B substrate, and 4 ug/ml Aurora B kinase.

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 16 mM glutathione

Aqueous buffer solution

>6 months at -80°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Delaval, B. et al., Oncogene 23 (26), 4516-4522 (2004).
2. Yasui, Y. et al., J. Biol. Chem. 279, 12997-13003 (2004).

Avoid freeze/thaw cycles

Kinase/Serine-Threonine

Recombinant Protein