TNK1 (Tyrosine Kinase Non Receptor 1), also known as CD38 Negative Kinase 1 (GenBank Accession No. NM_003985), a.a. 106-390, with N-terminal GST-tag and Thrombin Cleavage Site, MW = 57 kDa, expressed in Sf9 cells via a baculovirus expression system.

CD38 Negative Kinase 1, Tyrosine Kinase Non Receptor 1, Mitogen-Activated Protein Kinase 1, MAPK1, CD36 Molecule, CD36, RAF1

≥3.5 pmol/min/ug

Assay was done in Kinase buffer containing 1 mM DTT using CSKtide substrate (0.2 mg/ml) and 10 μM ATP. Reaction was done at 30°C for 50 min. Amount of ATP transferred was calculated using Kinase-Glo reagent (Promega)

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween20, 20% glycerol, 9mM Glutathione

Aqueous buffer solution

At least 6 months at –80°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Felschow, D. (2000). BBRC. 273(1):294-301.

2. Azoitei, N. et al. (2010). Oncogene. 26: 6536-6545.

Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 – 0.5%).

Kinase

Protein