Human TEK tyrosine kinase, endothelial, also known as Tie2, (GenBank Accession No. NM_000459) a.a. 771-1124(end) with N-terminal GST-tag, MW= 66 kDa, expressed in baculovirus infected Sf9 cell expression system.

Q02763

TIE, TEK, Tyrosine Kinase With Immunoglobulin-Like And EGF-Like Domains 1

1400 pmol/min/μg.

Enzyme reaction is conducted in a buffer containing 50 mM HEPES (pH 7.5), 10 mM MgCl₂, 1 mM EGTA, 200 μM ATP, 0.01% Brij-35, 2 μM substrate (Tyr Peptide 5, Z-lyte kinase assay kit from Invitrogen), and enzyme at 37°C for 1 hour.

40 mM Tris-HCl, pH 8, 110 mM NaCl, 2.2 mM KCl, 16 mM glutathione, 3 mM DTT, and 20% glycerol

Aqueous buffer solution

>6 months at –80°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Murray, B.W., et al., Biochemistry. 2001 Aug 28,40(34):10243-53.
2. Yuan, H.T., et al., Mol Cell Biol. 2009 Apr,29(8):2011-22.

Avoid freeze/thaw cycles

Kinase/Receptor Tyrosine

Recombinant Protein