| 产品名称 | ROR2, GST-tag - Kinase Receptor Tyrosine - Kinase(s) |
| 产品货号 | 40296 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | 10 µg |
| 产品品牌 | bpsbioscience |
| 产品概述 | |
| 产品详情 |
Species: Human
Host Species: Sf9 insect cells
MW: 86 kDa
Genbank #: NM_004560
Tag(s): N-terminal GST-tag
a.a: 427–end
Background: ROR2 is a receptor protein tyrosine kinase and type I transmembrane protein that belongs to the ROR subfamily of cell surface receptors. ROR2 is selectively expressed in the chondrocytes of all developing cartilage anlagen, where it is essential during initial growth and patterning, as well as subsequently in the proliferating chondrocytes of mature growth plates, where it is required for normal expansion (1). Thus, ROR2 is selectively expressed in and particularly important for the chondrocyte lineage and loss of ROR2 leads to profound skeletal abnormalities (2).
Description: Recombinant human ROR2 (a.a. 427-end), with N-terminal GST-tag, expressed by Sf9 insect cells via a baculovirus expression system.
Synonym(s): BDB, BDB1, NTRKR2, MGC163394, ROR
Purity: ≥80%
Specific Activity: 0.17 pmol/min/µg
Assay Conditions: 5 mM MOPS, 2.5 mM β-glycerophosphate, 5 mM MgCl2, 1 mM EGTA, 0.4 mM EDTA, 0.05 mM DTT, 2 mM ATP. Incubate ROR2 with 0.25 mg/ml Myelin Basic
Protein substrate and 50 µM [33P]-ATP at 30°C for 15 minutes, then spot reaction on phosphocellulose paper, fix in 1% phosphoric acid, and assay with a scintillation counter.
Formulation: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol.
Format: Aqueous buffer solution
Storage / Stability: >6 months at -80°C. Application(s): Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s): 1. DeChiara, T. M. et al: Ror2, encoding a receptor-like tyrosine kinase, is required for cartilage and growth plate development. Nature Genet. 24: 271-274, 2000.
2. Nomi, M. et al : Loss of mRor1 enhances the heart and skeletal abnormalities in mRor2-deficient mice: redundant and pleiotropic functions of mRor1 and mRor2 receptor tyrosine kinases. Molec. Cell. Biol. 21: 8329-8335, 2001. Warning(s): Avoid freeze/thaw cycles
Scientific Category: Kinase/Receptor Tyrosine
Product Type: Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
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