Human PYK2 (GenBank Accession No. U33284), full length with N-terminal His-tag, MW=120 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

CADTK, CAK beta, CAK-beta, CAKB, Calcium-dependent tyrosine kinase, Cell adhesion kinase beta, FADK 2, FADK2, FAK2, Focal adhesion kinase 2, PKB, Proline-rich tyrosine kinase 2, protein kinase B, protein tyrosine kinase 2 beta, PTK, PTK2B, PYK2, RAFTK, Re

5.6 pmol/min/μg.

ADP-Glo assay with 0.1 mg/ml poly-(Glu4:Tyr) as a substrate and 100 mM ATP at final concentration. Reaction was done for 60 minutes at 30°C. ADP-Glo reagents were added for 45 minutes at RT, and Kinase detection reagents for 55 minutes at RT, followed by luminescence detection.

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 200 mM imidazole

Aqueous buffer solution

>6 months at –80°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Cain R.J. et al., J Cell Biol. 2010 Mar 22,188(6):863-76.
2. Collins M. et al., Mol Immunol. 2010 May,47(9):1665-74.

Avoid freeze/thaw cycles

Kinase/Cytoplasmic Tyr

Recombinant Protein