| 产品名称 | Mouse EPHB1, GST-tag - Kinase Receptor Tyrosine - Kinase(s) |
| 产品货号 | 40199 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | 10 µg |
| 产品品牌 | bpsbioscience |
| 产品概述 | |
| 产品详情 |
Species: Mouse
Host Species: Sf9 insect cells
MW: 62 kDa
Genbank #: NM_173447
Tag(s): N-terminal GST-tag
a.a: 591–end
Background: EPHB1 is a member of the Eph family of receptor tyrosine kinases. Ligand-activated EPHB1 forms a signaling complex with Nck, paxillin, and focal adhesion kinase and induces tyrosine phosphorylation of paxillin in a c-Src-dependent manner to promote cell migration (1). In addition, activated EPHB1 recruits the adaptor proteins Grb2 and p52Shc and promotes p52Shc and c-Src tyrosine phosphorylation as well as MAPK/extracellular signal-regulated kinase (ERK) activation. Expression of dominant-negative c-Src significantly reduced EPHB1-dependent ERK1/2 activation and chemotaxis (2).
Description: Recombinant mouse EPHB1 (a.a. 591-end), with N-terminal GST-tag, expressed by Sf9 insect cells via a baculovirus expression system.
Synonym(s): Elk, Net, Cek6, Elkh, Hek6, EPHT2, AW488255, 9330129L11, Ephrin Type B Receptor 2
Purity: ≥90%
Formulation: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol.
Format: Aqueous buffer solution
Storage / Stability: >6 months at -80°C. Application(s): Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s): 1. Vindis, C. et al: EphB1-mediated cell migration requires the phosphorylation of paxillin at Tyr-31/Tyr-118. J Biol Chem. 2004 Jul 2;279(27):27965-70.
2. Vindis, C. et al: EphB1 recruits c-Src and p52Shc to activate MAPK/ERK and promote chemotaxis. J Cell Biol. 2003 Aug 18;162(4):661-71. Warning(s): Avoid freeze/thaw cycles
Scientific Category: Kinase/Receptor Tyrosine
Product Type: Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
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