Human Jak1 (Janus kinase 1), also known as JTK3, GenBank Accession No. NM_002227, a.a. 866-1154(end) with N-terminal GST-Tag, MW= 59 kDa, expressed in Sf9 cells via a baculovirus expression system.

P23458

JAK1, Janus kinase-1, Just Another Kinase 1, JTK3, jak

>60 pmole/min/ug

Assay was done in Kinase buffer containing 2 mM DTT using IRS-1 derived peptide substrate (0.1 mg/ml) and 20 µM ATP. Reaction was done at 30°C for 45 min. Amount of ATP transferred was calculated using Kinase-Glo reagent (Promega).

40 mM Tris-HCl pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 16 mM glutathione and 20% glycerol.

Aqueous buffer solution

At least 6 months at –80°C

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Rodig, S.J., et al. (1998). Cell 93 (3): 373–8.
2. Haan, C., et al. (2001). J. Biol. Chem. 276 (40): 37451–8.
3. Miyazaki, T., et al. (1994). Science 266 (5187): 1045–7

Avoid freeze/thaw cycles

Kinase/Cytoplasmic Tyr

Recombinant Protein