Human ITK (GenBank Accession No. NM_005546), a.a. 352-end, with N-terminal GST-tag, MW= 57 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

ITK, IL2-inducible T-cell kinase , Lyk, TSK, ITK

13 pmol/min/μg

50 mM HEPES (pH 7.5), 10 mM MgCl₂, 1 mM EGTA, 0.01% Brij-35, 200 μM ATP, 2 μM Tyr1 substrate (Z-lyte kinase assay kit from Invitrogen), 1 hour at room temperature.

25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 1.37 mM KCl, 0.05% Tween-20, 30% glycerol, 10 mM glutathione, and 3 mM DTT.

Aqueous buffer solution

>6 months at –80°C

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. August, A. et al. Int J Biochem Cell Biol. 2002 Oct,34(10):1184-9.
2.Finkelstein, L D. et al. Trends Cell Biol. 2004 Aug,14(8):443-51.

Avoid freeze/thaw cycles

Kinase/Cytoplasmic Tyr

Recombinant Protein