| 产品名称 | EPHA4, GST-tag - Kinase Receptor Tyrosine - Kinase(s) |
| 产品货号 | 40193 |
| 产品价格 | 现货询价，电话：010-67529703 |
| 产品规格 | 10 µg |
| 产品品牌 | bpsbioscience |
| 产品概述 | |
| 产品详情 |
Species: Human
Host Species: Sf9 insect cells
MW: 58 kDa
Genbank #: NM_004438
Tag(s): N-terminal GST-tag
a.a: 610–887
Background: EPHA4 also known as EPH receptor A4, belongs to the ephrin receptor subfamily of protein-tyrosine kinases which have been implicated in mediating developmental events, particularly in the nervous system (1). The EPHA4 ligand ephrin-A3 is localized to the astrocytic processes that envelop the spine. Activation of EPHA4 by ephrin-A3 induces spinal retraction and reduces spine density and inhibits the interaction distorted spine shape and organization. EPHA4-null mice possess defects in the corticospinal tract and anterior commissure indicating a model in which an ephrin ligand on the axons senses EPHA4 on spinal cord cells surrounding the corticospinal tract (2).
Description: Human EPHA4 (Ephrin Receptor A4), also known as TYRO1, SEK, and HEK8, GenBank Accession No. NM_004438, a.a. 610-887, with N-terminal GST-tag, MW ~58 kDa, expressed in Sf9 insect cells via a baculovirus expression system.
Synonym(s): SEK, HEK8, TYRO1
Purity: ≥90%
Specific Activity: 61 pmol/min/µg
Assay Conditions: Assay was done in Kinase buffer containing 0.2 mM DTT using Poly-(Glu4:Tyr)-biotin substrate (0.2 mg/ml) and 20 µM ATP. Reaction was done at 30°C for 40 min. Amount of ATP transferred was calculated using Kinase-Glo reagent (Promega). Formulation: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.
Format: Aqueous buffer solution
Storage / Stability: >6 months at -80°C. Application(s): Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s): 1. Flanagan, J.G. et al: The ephrins and Eph receptors in neural development.". Annu. Rev. Neurosci. 1998;21: 309–45.
2. Dottori, M. et al: EphA4 (Sek1) receptor tyrosine kinase is required for the development of the corticospinal tract. Proc. Nat. Acad. Sci. 95: 13248-13253, 1998. Warning(s): Avoid freeze/thaw cycles
Scientific Category: Kinase/Receptor Tyrosine
Product Type: Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
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