Human EGFR with T790M mutation also known as, Epidermal Growth Factor Receptor, ERBB, ErbB-1, HER1, and MENA, GenBank Accession No. NM_005228, a.a. 668-1210 with C-terminal His-tag and N-terminal GST-tag, expressed in Sf9 cells via a baculovirus expression system. MW = 88 kDa.

P00533

EGFR, Epidermal Growth Factor Receptor, ERBB, ErbB-1, HER1, MENA

113 pmol/min/μg.

40 mM Tris-HCl pH 7.4, 20 mM MgCl2, 0.1 mg/mL BSA and 0.2 mM DTT using 0.2 mg/ml Poly-(Glu4:Tyr)-biotin substrate and 20 mM ATP. Reaction was done at 30°C for 35 min. Amount of ATP transferred was calculated using Kinase-Glo reagent from Promega.

25 mM Tris-HCl, pH 8.0, 138 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.

Aqueous buffer solution

At least 6 months at –80°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Adachi,S. et al., Cancer Res. 67 (13), 6493-6501 (2007)
2. Rosano,L. et al., Cancer Res. 67 (13), 6351-6359 (2007).

Application Reference:
Figueroa, M., et al., J. Antibiotics. 65, 559-564 (2012).

Avoid freeze/thaw cycles

Kinase/Receptor Tyrosine

Recombinant Protein