Complex of N-terminal FLAGtagged recombinant full-length human p110 (GenBank Accession No. U79143), with K111N mutation, and recombinant full length, human p85 (no tag, GenBank Accession No. XM_043865). Co-expressed in Sf9 cells in a Baculovirus expression system. p110(K111N) MW=124 kDa, p85 MW=84 kDa.

PIK3CA, p110alpha, Phosphoinositide-3-Kinase, Catalytic, Alpha, PIK3R1, Phosphoinositide-3-Kinase, Regulatory Subunit 1 (Alpha), GRB1, p85alpha

1326 pmol/min/μg.

Reactions (25 μl) were done at 30°C for 15 min using BPS PI3K substrate, and the samples were incubated with the ADPGlo reagent (25 μl) for 45 min followed by incubation with the Kinase Detection reagent (50 μl) for 55 min.

40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, 0.2 mM TCEP, 80 μg/ml flag peptide

Aqueous buffer solution

At least 6 months at –80°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Rudd, M.L., et al., Clin. Canc. Res. 2011; 17(6): 1331-1340.
2. Brady, S.W., et al., Molec. Canc. Ther. 2014; 13(1): 60-70.

Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 – 0.5%).

Kinase

Lipid