Proto-oncogene tyrosine-protein kinase YES1 has been implicated in regulation of cell growth and survival, apoptosis, cell-cell adhesion, and differentiation. It has been identified as a potential target in basal-like breast cancers. The YES1 Assay Kit is designed to measure YES1 activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent. The YES1 Assay Kit comes in a convenient 96-well format, with enough purified recombinant YES1 enzyme, Protein Tyrosine Kinase Substrate (Poly-Glu,Tyr 4:1), ATP, and kinase assay buffer for 100 enzyme reactions.

Kinase-Glo MAX (Promega #V6071)

Dithiothreitol (DTT, 1 M; optional)

Microplate reader capable of reading luminescence

Adjustable micropipettor and sterile tips

30°C incubator

HsT441, P61-YES, Yes, c-yes, YES proto-oncogene 1, Src family tyrosine kinase

See assay kit data sheet for detailed protocol

Up to 6 months when stored as recommended.

Useful for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.

1. Hamamura K., Tsuji M., et al. Functional Activation of Src Family Kinase Yes Protein is Essential for the Enhanced Malignant Properties of Human Melanoma Cells Expressing Ganglioside GD3; 2011, J.] Biol. Chem.] 286: 18526-18537.

2. Bilal, E., Alexe G., et al. Identification of the YES1 Kinase as a Therapeutic Target in Basal-Like Breast Cancers; 2010, Genes & Cancer Oct; 1(10): 1063–1073.

Avoid multiple freeze/thaw cycles.

Kinase

Assay Kit