Janus kinase (JAK) is a family of intracellular, nonreceptor tyrosine kinases that play an important role in cytokine-mediated signaling. This family includes four protein kinases; Jak1, Jak2, Jak3 and Tyk2. Various studies have suggested that regulation of Jak activity should be a promising approach to combat numerous diseases such as immune deficiencies and cancers. The JAK3 (Janus Kinase 3) Assay Kit is designed to measure JAK3 activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent.

Kinase-Glo MAX (Promega #V6071)

Dithiothreitol (DTT, 1 M; optional)

Microplate reader capable of reading luminescence

Adjustable micropipettor and sterile tips

30°C incubator

jak, just another kinase

The JAK3 (Janus Kinase 3) Assay Kit comes in a convenient 96-well format, with enough purified recombinant JAK3 enzyme, JAK3 substrate peptide (Poly(Glu;Tyr, 4:1)), ATP and kinase assay buffer for 100 enzyme reactions.

See assay kit data sheet for detailed protocol

Up to 6 months when stored as recommended.

Useful for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.

Alicea, N.L. et al., Current Drug Targets 12(4):546-555 (2011)

Avoid freeze/thaw cycles

Kinase

Assay Kit