The Tyrosine Phosphorylation Assay Kit is designed to measure TYR phosphorylation of proteins. The key to the Tyrosine Phosphorylation Assay Kit is a specific antibody mixture that provides sequence-independent recognition of phosphor-TYR residues. With this kit, only three simple steps on a microtiter plate are required for detection of the phosphorylation state. First, the protein of interest is coated on the strip plate. Next, primary antibody is added. Finally, the strips are treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.

Protein of interest

TBST buffer (1x Tris-buffered saline, pH 8.0, containing 0.05% Tween-20)

Luminometer or fluorescent microplate reader capable of reading chemiluminescence

Rotating or rocker platform

Adjustable micropipettor and sterile tips

See assay kit data sheet for detailed protocol

Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

Great for detection of tyrosine phosphorylation of recombinant proteins. Useful to examine the change of tyrosine phosphorylation level in response to various treatments.

Wang, J.Y.J. 1988. Antibodies for phosphotyrosine: Analytical and preparative tool for tyrosyl-phosphorylated proteins. Anal. Biochem.172(1): 1-7.

Avoid freeze/thaw cycles

Kinase

Assay Kit