Human TRKA, also known as NTKR1, and Neurotrophic Tyrosine Kinase Receptor, type 1, GenBank Accession No. NM_002529, a.a. 442-796(end), with Nterminal GST-tag, expressed in Sf9 cells via a baculovirus expression system. MW=65 kDa.

P04629

TRKA, NTKR1, Neurotrophic Tyrosine Kinase Receptor, type 1

Aqueous buffer solution.

100 pmole/min/μg

40 mM Tris-HCl pH 7.4, 20 mM MgCl2, 0.1 mg/mL BSA and 1 mM DTT using 0.2 mg/ml Poly(Glu:Tyr) substrate and 20 μM ATP. Reaction was done at 30°C for 45 min. Amount of ATP transferred was calculated using Kinase-Glo reagent from Promega.

40 mM Tris-HCl pH8.0, 110mM NaCl, 2.2 mM KCl,0.04% Tween20, 3mM DTT, and 20% glycerol

At least 6 months at –80°C.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

1. Koch, A., et al. (2000). FEBS Lett. 469 (1): 72–62.
2. Wiesmann, C., et al. (1999). Nature 401 (6749): 184–83.
3. Yamashita, H. et al. (1999). J. Biol. Chem. 274 (21): 15059–65

Avoid freeze/thaw cycles

Kinase/Receptor Tyrosine

Recombinant Protein